ISO (International Organization for Standardization) for a digital camera is the measurement of the sensitivity of the image sensor. The higher the ISO number the more sensitive your sensor will be to light resulting in more noise or grain in your image, and the lower the ISO number the less sensitive your sensor will be to light resulting in finer noise or grain in your image.
A reason why you may want to use a higher ISO would be if you are shooting in low light and need a faster shutter speed to freeze action such as at a sporting event. The following images were taken using a Canon 50D to show ISO in low light, normal light and night light.
LOW LIGHT
NORMAL LIGHT
NIGHT LIGHT
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